Composite
Syn_Fuel

Part:BBa_K610000:Design

Designed by: Choi JinSik   Group: iGEM11_Korea_U_Seoul   (2011-10-05)

alkane synthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3169
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2567
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 4492


Design Notes

Alkane sythesis. Lux A, B are removed in lux system. And their are substituted by FAD(fatty aldehyde decarbonylase) gene.

Source

Luminescence system.

References

Edward A.. Meighen. 1988 ENZYMES AND GENES FROM THE lux OPERONS OF BIOLUMINESCENT BACTERIA. Annu. Rev. Microbiol.42:151-76